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Data S3. Validation of the A(H5) antigenic map. Interactive versions of the three-dimensional antigenic map, represented as described for Data S2. (A, B) Piecewise Procrustes analysis (see fig. S2 and detailed in the supplementary text) comparing the antigenic maps in three and four dimensions. The results of analysis with one (A) and two (B) pieces are displayed. The antigen color hue indicated which piece it belongs to, and the shading indicates the Procrustes distance according to the gradient displayed on the right, in antigenic units (AU). (C) Triangulation blobs indicating the area in which each datapoint can be located in the antigenic map without increasing the total map stress by more than one unit. (D) Alternative antigenic map conformation found upon comparing all 1000 optimizations (see fig. S4 and supplementary text 3). The map from optimization 602 is shown. (E) The lowest stress antigenic map (optimization 1), with Procrustes arrows pointing towards the positions of each antigen and serum in the optimization 602 map conformation. (F) Bayesian bootstrap blob size analysis. The antigen color corresponds to the radius (AU) of a sphere of equal volume than each blob as displayed on the right. For interpretation, 1-2 AU differences correspond to the assay variation.